Methods

• Flow Cytometry
• Protein isolation, -quantification and Western Blot
• Tissue embedding (cryo und Paraffin)
• Immunohistochemistry
• Immunohistology
• Immunofluorescence
• ELISA
• Hormone Measurements
• DNA Isolation (from cells and tissue)
• RNA Isolation (from cells and tissue)
• Classic PCR
• Quantitative PCR (SybrGreen and TaqMan Probe)
• Transduction and Transfection (Retrovirus)
• Isolation of cell populations (Treg, DCs, CD4+ cells)
• Cell culture (cell lines and primary cultures)
• Proliferation assays
• Migration assays
• siRNA transfection
• Murine models for miscarriage, pre-eclampsia, pseudo-pregnancy
• In vivo experiments (mouse): i.p., i.v. injections, immunization, cell transfer, blood pressure measurement, measurement of physiological parameters, gene therapy, in vitro fertilization (IVF), hormonal stimulation