Methodenspektrum

  • Flow Cytometry
  • Protein isolation, -quantification and Western Blot
  • Tissue embedding (cryo und Paraffin)
  • Immunohistochemistry
  • Immunohistology
  • Immunofluorescence
  • ELISA
  • Hormone Measurements
  • DNA Isolation (from cells and tissue)
  • RNA Isolation (from cells and tissue)
  • Classic PCR
  • Quantitative PCR (SybrGreen and TaqMan Probe)
  • Transduction and Transfection (Retrovirus)
  • Isolation of cell populations (Treg, DCs, CD4+ cells)
  • Cell culture (cell lines and primary cultures)
  • Proliferation assays
  • Migration assays
  • siRNA transfection
  • Murine models for miscarriage, pre-eclampsia, pseudo-pregnancy
  • In vivo experiments (mouse): i.p., i.v. injections, immunization, cell transfer, blood pressure measurement, measurement of physiological parameters, gene therapy, in vitro fertilization (IVF), hormonal stimulation